Микробиология

Phosphorylation of nuclear and cytoplasmic pools of ribosomal protein S6 during cell cycle progression

SHORT COMMUNICATION Margit Rosner • Katharina Schipany • Markus Hengstschla ¨ger Received: 28 September 2012 / Accepted: 7 December 2012 / Published online: 20 December 2012 Springer-Verlag Wien 2012 Abstract Of all known ribosomal proteins, the 40S ribosomal protein S6 is by far the most extensively studied. Still, little is known about some basic aspects of S6 regulation including its cell cycle-related expression and localization. Using a flow cytometric single cell approach applied to whole cells and isolated nuclei, we monitored

Simultaneous analysis of the non-canonical amino acids norleucine and norvaline in biopharmaceutical-related fermentation processes by a new ultra-high performance liquid chromatography approach

ORIGINAL ARTICLE Michael Biermann • Bettina Bardl • Sebastian Vollsta ¨dt • Julia Linnemann • Uwe Knu ¨pfer • Guido Seidel • Uwe Horn Received: 29 December 2012 / Accepted: 2 January 2013 / Published online: 11 January 2013 The Author(s) 2013. This article is published with open access at Springerlink.com Abstract In this study, a precise and reliable ultra-high performance liquid chromatography (UHPLC) method for the simultaneous determination of non-canonical (norvaline and norleucine) and standard amino acids (aspartic acid, glutamic acid, serine, histidine, glycine, threonine,

Rational design of cationic antimicrobial peptides by the tandem of leucine-rich repeat

ORIGINAL ARTICLE Q. Q. Ma • Y. F. Lv • Y. Gu • N. Dong • D. S. Li • A. S. Shan Received: 14 September 2012 / Accepted: 28 December 2012 / Published online: 22 February 2013 Springer-Verlag Wien 2013 Abstract Antimicrobial peptides represent ancient host defense effector molecules present in organisms across the evolutionary spectrum. Lots of antimicrobial peptides were synthesized based on well-known structural motif widely existed in a variety of lives. Leucine-rich repeats (LRRs)

A quantitative analysis of spontaneous isoaspartate formation from N-terminal asparaginyl and aspartyl residues

ORIGINAL ARTICLE Bert H.-O. Gu ¨ttler • Holger Cynis • Franziska Seifert • Hans-Henning Ludwig • Andrea Porzel • Stephan Schilling Received: 18 September 2012 / Accepted: 24 December 2012 / Published online: 24 January 2013 Springer-Verlag Wien 2013 Abstract The formation of isoaspartate (isoAsp) from asparaginyl or aspartyl residues is a spontaneous posttranslational modification of peptides and proteins. Due to isopeptide bond formation, the structure and possibly function of peptides and proteins is altered. IsoAsp modifications within the peptide chain have been reported for

Ant 4,4, a polyamine-anthracene conjugate, induces cell death and recovery in human promyelogenous leukemia cells (HL-60)

ORIGINAL ARTICLE Rui Traquete • Radiah A. Ghani • Otto Phanstiel • Heather M. Wallace Received: 14 November 2012 / Accepted: 21 December 2012 / Published online: 6 January 2013 Springer-Verlag Wien 2013 Abstract One of the major problems in cancer therapy is the lack of specificity of chemotherapeutic agents towards cancer cells, resulting in adverse side effects. One means to counter this is to selectively deliver the drug to the cancer cell. Cancer cells accumulate increased concentrations of polyamines compared to normal cells, mainly through an

Chemical rescue of the post-translationally carboxylated lysine mutant of allantoinase and dihydroorotase by metal ions and short-chain carboxylic acids

ORIGINAL ARTICLE Ya-Yeh Ho • Yen-Hua Huang • Cheng-Yang Huang Received: 22 October 2012 / Accepted: 21 December 2012 / Published online: 4 January 2013 Springer-Verlag Wien 2013 Abstract Bacterial allantoinase (ALLase) and dihydroorotase (DHOase) are members of the cyclic amidohydrolase family. ALLase and DHOase possess similar binuclear metal centers in the active site in which two metals are bridged by a post-translationally carboxylated lysine. In this study, we determined the effects of carboxylated lysine and metal binding on the activities of ALLase and DHOase. Although

4-[18F]Fluoro-N-methyl-N-(propyl-2-yn-1-yl)benzenesulfonamide ([18F]F-SA): a versatile building block for labeling of peptides, proteins and oligonucleotides with fluorine-18 via Cu(I)-mediated click chemistry

ORIGINAL ARTICLE Theres Ramenda • Jo ¨rg Steinbach • Frank Wuest Received: 6 September 2012 / Accepted: 21 December 2012 / Published online: 10 January 2013 Springer-Verlag Wien 2013 Abstract Cu(I)-mediated [3?2]cycloaddition between azides and alkynes has evolved into a valuable bioconjugation tool in radiopharmaceutical chemistry. We have developed a simple, convenient and reliable radiosynthesis of 4-[18F]fluoroN-methyl-N-(propyl-2-yn-1-yl)benzenesulfonamide ([18F]FSA) as a novel aromatic sulfonamide-based click chemistry building block. [18F]F-SA could be prepared in a remotely

A novel approach in potential anticoagulants from peptides epitope 558–565 of A2 subunit of factor VIII

ORIGINAL ARTICLE C. Anastasopoulos • Y. Sarigiannis • G. Stavropoulos Received: 5 October 2012 / Accepted: 14 December 2012 / Published online: 9 January 2013 Springer-Verlag Wien 2013 Abstract Factor VIII, a human blood plasma protein, plays an important role during the intrinsic pathway of blood coagulation cascade after its activation by thrombin. The activated form of FVIII acts as cofactor to the serine protease Factor IXa, in the conversion of the zymogen Factor X to the active enzyme Factor Xa. The Ser558–Gln565 region of the A2 subunit of Factor VIII has been shown to be crucial for

Astroglial amino acid-based transmitter receptors

INVITED REVIEW Vladimir Parpura • Alexei Verkhratsky Received: 31 December 2012 / Accepted: 2 January 2013 / Published online: 26 January 2013 Springer-Verlag Wien 2013 Abstract Amino acids appear in prebiotic period being one of the first organic molecules on Earth. For neurobiologists, it is of importance that AAs are not only representing building blocks of life, but are also the essential part of metabolism and cellular signaling. In the mammalian brain, the most common excitatory and inhibitory transmitters acting upon cellular plasmalemmal receptors

Potency of GABA at human recombinant GABAA receptors expressed in Xenopus oocytes: a mini review

INVITED REVIEW Nasiara Karim • Petrine Wellendorph • Nathan Absalom • Graham A. R. Johnston • Jane R. Hanrahan • Mary Chebib Received: 23 December 2012 / Accepted: 26 December 2012 / Published online: 6 February 2013 Springer-Verlag Wien 2013 Abstract GABAA receptors are members of the ligandgated ion channel superfamily that mediate inhibitory neurotransmission in the central nervous system. They are thought to be composed of 2 alpha (a), 2 beta (b) subunits and one other such as a gamma (c) or delta (d) subunit. The

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