Биология

Comparison of molecular markers to detect fresh sewage in environmental waters

W. Ahmed a,b,*, A. Goonetilleke b, D. Powell c, K. Chauhan b, T. Gardner a,b a Department of Natural Resources and Water, 80 Meiers Road, Indooroopilly, Brisbane 4068, Australia b School of Urban Development, Queensland University of Technology, GPO Box 2434, Brisbane 4001, Australia c Faculty of Science, Health and Education, University of the Sunshine Coast, Maroochydore DC, 4558, Qld, Australia a r t i c l e i n f o Article history: Received 2 March 2009 Received in revised form 26 July 2009 Accepted 17 September 2009 Published online 22 September 2009 Keywords: Microbial source tracking

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Evaluation of rapid methods and novel indicators for assessing microbiological beach water quality

John F. Griffith a,*, Yiping Cao a, Charles D. McGee b, Stephen B. Weisberg a a Southern California Coastal Water Research Project, Costa Mesa, CA, United States b Orange County Sanitation District, Fountain Valley, CA, United States a r t i c l e i n f o Article history: Received 13 May 2009 Received in revised form 24 August 2009 Accepted 7 September 2009 Published online 8 September 2009 Keywords: Water quality indicators Fecal indicator bacteria Method evaluation a b s t r a c t A broad suite of new measurement methods and indicators based on molecular

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Evaluating the operational utility of a Bacteroidales quantitative PCR-based MST approach in determining the source of faecal indicator organisms at a UK bathing water

Carl M. Stapleton a, David Kay a,*, Mark D. Wyer a, Cheryl Davies b, John Watkins b, Chris Kay a, Adrian T. McDonald c, Jonathan Porter d, Andrew Gawler d aCREH, Catchment and Coastal Research Centre, River Basin Dynamics and Hydrology Research Group, IGES, University of Aberystwyth, Ceredigion, SY23 3DB, UK bCREH Analytical, Hoyland House, 50 Back Lane, Horsforth, Leeds LS18 4RS, UK cFaculty of the Environment, University of Leeds, Leeds, LS2 9JT, UK dEnvironment Agency, National Laboratory Service, Starcross Laboratory, Starcross, Exeter, Devon, EX6 8PE, UK a r t i c l e i n f o

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Evaluation of the enterococci indicator in biosolids using culture-based and quantitative PCR assays

Emily Viau, Jordan Peccia* Department of Chemical Engineering, Environmental Engineering Program, Yale University, New Haven, CT 06520, USA a r t i c l e i n f o Article history: Received 28 May 2009 Received in revised form 18 August 2009 Accepted 6 September 2009 Published online 11 September 2009 Keywords: Sewage sludge Real-time PCR Fecal streptococcus VBNC Compost Pathogen a b s t r a c t The utility of the enterococci indicator for measuring biosolids quality was evaluated in biosolids from 22 U.S. wastewater treatment facilities. Enterococci were enumerated using

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Evaluation of multiple sewage-associated Bacteroides PCR markers for sewage pollution tracking

W. Ahmed a,b,*, A. Goonetilleke b, D. Powell b, T. Gardner a,b a Department of Natural Resources and Water, 80 Meiers Road, Indooroopilly, Sunshine Coast, QLD 4558, Brisbane 4068, Australia b School of Urban Development, Queensland University of Technology, GPO Box 2434, Brisbane 4001, Australia a r t i c l e i n f o Article history: Received 13 March 2009 Received in revised form 20 August 2009 Accepted 30 August 2009 Published online 3 September 2009 Keywords: Feral pollution Water quality Microbial source tracking Bacteroides Sewage-associated markers a b s t r a c t

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Concentrations of host-specific and generic fecal markers measured by quantitative PCR in raw sewage and fresh animal feces

Sarah S. Silkie, Kara L. Nelson* Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720-1710, USA a r t i c l e i n f o Article history: Received 6 March 2009 Received in revised form 5 June 2009 Accepted 8 August 2009 Published online 22 August 2009 Keywords: Quantitative PCR Fecal source tracking Bacteroidales Fecal indicator bacteria DNA recovery Host-specific marker a b s t r a c t

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Rapid decay of host-specific fecal Bacteroidales cells in seawater as measured by quantitative PCR with propidium monoazide

Sungwoo Bae, Stefan Wuertz* Department of Civil and Environmental Engineering, 2001 EU III, University of California, Davis, One Shields Avenue, Davis, CA 95616, USA a r t i c l e i n f o Article history: Received 20 March 2009 Received in revised form 22 June 2009 Accepted 28 June 2009 Published online 3 July 2009 Keywords: Persistence and survival Host-specific Bacteroidales Microbial source tracking Fecal pollution Propidium monoazide a b s t r a c t We investigated the persistence of feces-derived Bacteroidales cells and their DNA in

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Evaluation of host-specific Bacteroidales 16S rRNA gene markers as a complementary tool for detecting fecal pollution in a prairie watershed

B. Fremaux, J. Gritzfeld, T. Boa, C.K. Yost* Laboratory for Irrigation Water Protection, Biology Department, University of Regina, 3737 Wascana Parkway, Regina, SK S4S0A2, Canada a r t i c l e i n f o Article history: Received 19 February 2009 Received in revised form 12 June 2009 Accepted 22 June 2009 Published online 27 June 2009 Keywords: Bacteroidales 16S rRNA gene markers Bacterial source tracking Fresh water Irrigation Fecal pollution Enteric pathogens a b s t r a c t Our ability to identify and eliminate fecal contamination of water, now and in the future, is

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Rapid QPCR-based assay for fecal Bacteroides spp. as a tool for assessing fecal contamination in recreational waters

Reagan R. Conversea,*, A. Denene Blackwooda, Marek Kirsb, John F. Griffithc, Rachel T. Noblea aInstitute of Marine Sciences, University of North Carolina at Chapel Hill, 3431 Arendell St., Morehead City, NC 28557, USA bCawthron Institute, 98 Halifax St. East, Nelson 7042, New Zealand cSouthern California Coastal Water Research Project, 3535 Harbor Blvd., Costa Mesa, CA 92626, USA a r t i c l e i n f o Article history: Received 15 March 2009 Received in revised form 8 June 2009 Accepted 10 June 2009 Published online 24 June 2009 Keywords: Bacteroides Enterococcus speciation

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Evaluation of two spike-and-recovery controls for assessment of extraction efficiency in microbial source tracking studies

Donald M. Stoeckela, Erin A. Stelzera, Linda K. Dickb,* aUS Geological Survey, Ohio Water Microbiology Laboratory, Columbus, OH 43229, USA bSchool of Environment and Natural Resources, The Ohio State University, Columbus, OH 43210, USA a r t i c l e i n f o Article history: Received 10 March 2009 Received in revised form 4 June 2009 Accepted 10 June 2009 Published online 21 June 2009 Keywords: Quality control Quantitative polymerase chain reaction Extraction efficiency Water Microbial source tracking a b s t r a c t

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