Биохимия

Inflammatory serum proteome pattern in mice fed a high-fat diet

ORIGINAL ARTICLE Elisabetta Gianazza • Cristina Sensi • Ivano Eberini • Federica Gilardi • Marco Giudici • Maurizio Crestani Received: 16 October 2012 / Accepted: 17 November 2012 / Published online: 6 December 2012 Springer-Verlag Wien 2012 Abstract To investigate the influence of diet on serum protein pattern, mice were fed for 8 weeks either control chow or a high-fat diet (containing 21 % w/w milk fat and 0.2 % w/w cholesterol); sera were collected and analyzed by 2-DE. The main positive acute-phase reactant proteins,

Preference toward a polylysine enantiomer in inhibiting prions

ORIGINAL ARTICLE Karen S. Jackson • Jihyun Yeom • Youngmi Han • Younsoo Bae • Chongsuk Ryou Received: 30 July 2012 / Accepted: 6 November 2012 / Published online: 21 November 2012 Springer-Verlag Wien 2012 Abstract Differential anti-prion activity of polylysine enantiomers was studied. Based on our recent discovery that poly-L-lysine (PLK) is a potent anti-prion agent, we investigated suppression of prions in cultured cells using poly-D-lysine (PDK). The results showed that PDK was

Acute tryptophan depletion does not improve endurance cycling capacity in a warm environment

ORIGINAL ARTICLE Ruth M. Hobson • Phillip Watson • Ronald J. Maughan Received: 10 July 2012 / Accepted: 6 November 2012 / Published online: 21 November 2012 Springer-Verlag Wien 2012 Abstract Newsholme’s theory of central fatigue suggests that acute tryptophan depletion should improve endurance exercise capacity in a warm environment by reducing serotonergic activity in the brain. Eight males cycled to volitional exhaustion at 55 % V _O2 peak in 30.1 ± 0.5 C and 30 ± 7 % relative humidity on two separate occasions, after consuming either an amino acid load to deplete their

Synthesis of gem-diamino acid derivatives by a Hofmann rearrangement

ORIGINAL ARTICLE Emanuele Aresu • Stefania Fioravanti • Simona Gasbarri • Lucio Pellacani • Federico Ramadori Received: 26 September 2012 / Accepted: 4 November 2012 / Published online: 22 November 2012 Springer-Verlag Wien 2012 Abstract Starting from commercially available N-protected L-a-amino acids, N,N0-protected gem-diaminic units were obtained by a two-step methodology. A Hofmann reaction performed using a primary alcohol as the solvent to trap the isocyanate intermediate represents the key step of the new synthetic procedure. Then, the methodology

Combination of BCAAs and glutamine enhances dermal collagen protein synthesis in protein-malnourished rats

ORIGINAL ARTICLE Hitoshi Murakami • Kazutaka Shimbo • Yoshinobu Takino • Hisamine Kobayashi Received: 6 September 2012 / Accepted: 25 October 2012 / Published online: 8 November 2012 Springer-Verlag Wien 2012 Abstract Skin collagen decreases in protein-malnourished states. Amino acids regulate protein metabolism, glutamine stimulates collagen synthesis through the conversion process to proline and provides 75 % of the intracellular free proline in fibroblasts. However, the impact of these amino acids on collagen synthesis under malnutrition has not been

Vaspin attenuates the apoptosis of human osteoblasts through ERK signaling pathway

ORIGINAL ARTICLE Xiao Zhu • Yi Jiang • Peng-Fei Shan • Jie Shen • Qiu-Hua Liang • Rong-Rong Cui • Yuan Liu • Guan-Ying Liu • Shan-Shan Wu • Qiong Lu • Hui Xie • You-Shuo Liu • Ling-Qing Yuan • Er-Yuan Liao Received: 6 July 2012 / Accepted: 22 October 2012 / Published online: 8 November 2012 Springer-Verlag Wien 2012 Abstract It has been hypothesized that adipocytokines originating from adipose tissue may have an important role in bone metabolism. Vaspin is a novel adipocytokine isolated from visceral white adipose tissue, which has been

PEGylated dendrimer polystyrene support: synthesis, characterisation and evaluation of biologically active peptides

ORIGINAL ARTICLE M. A. Siyad • G. S. Vinod Kumar Received: 17 June 2012 / Accepted: 12 October 2012 / Published online: 1 November 2012 Springer-Verlag Wien 2012 Abstract Poly(N,N-bisethylamine) dendrimers with high content of poly(ethylene glycol) were synthesized on 3-(Acryloyloxy)-2-hydroxypropylmethacrylate-crosslinked polystyrene (PS-AHMA) resin and tested in various conditions of solid phase peptide synthesis. The dendritic templates were generated to the second generation on

Synthesis, characterization, conformational analysis of a cyclic conjugated octreotate peptide and biological evaluation of 99mTc-HYNIC-His3-Octreotate as novel tracer for the imaging of somatostatin receptor-positive tumors

ORIGINAL ARTICLE Ashok Behera • Indranil Banerjee • Kakali De • Rudra Narayan Munda • Sankha Chattopadhayay • Amalesh Samanta • Bharat Sarkar • Santanu Ganguly • Mridula Misra Received: 28 June 2012 / Accepted: 12 October 2012 / Published online: 23 October 2012 Springer-Verlag Wien 2012 Abstract Peptides are attracting increasing interest in nuclear oncology for targeted tumor diagnosis and therapy. We therefore synthesized new cyclic octapeptides conjugated with HYNIC by Fmoc solid-phase peptide synthesis. These were purified and analyzed by RP-HPLC, MALDI

Glycine reduces platelet aggregation

ORIGINAL ARTICLE Peter Schemmer • Zhi Zhong • Uwe Galli • Michael D. Wheeler • Li Xiangli • Blair U. Bradford • Lars O. Conzelmann • Dow Forman • Jose ´ Boyer • Ronald G. Thurman Received: 7 August 2012 / Accepted: 11 October 2012 / Published online: 8 November 2012 Springer-Verlag Wien 2012 Abstract It has been demonstrated that a wide variety of white blood cells and macrophages (i.e. Kupffer cells, alveolar and peritoneal macrophages and neutrophils) contain glycine-gated chloride channels. Binding of glycine on the receptor stimulates Cl- influx causing membrane

Dietary supplementation with monosodium glutamate is safe and improves growth performance in postweaning pigs

ORIGINAL ARTICLE Reza Rezaei • Darrell A. Knabe • Carmen D. Tekwe • Sudath Dahanayaka • Martin D. Ficken • Susan E. Fielder • Sarah J. Eide • Sandra L. Lovering • Guoyao Wu Received: 5 October 2012 / Accepted: 9 October 2012 / Published online: 2 November 2012 Springer-Verlag Wien 2012 Abstract Dietary intake of glutamate by postweaning pigs is markedly reduced due to low feed consumption. This study was conducted to determine the safety and efficacy of dietary supplementation with monosodium glutamate (MSG) in postweaning pigs. Piglets were weaned at 21 days of age to a

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