Биохимия

Modulation of immune responses by the neuropeptide CGRP

INVITED REVIEW Bernhard Holzmann Received: 28 July 2011 / Accepted: 14 November 2011 / Published online: 24 November 2011 Springer-Verlag 2011 Abstract The peripheral nervous system is connected with lymphoid organs through sensory nerves that mediate pain reflexes and may influence immune responses through the release of neuropeptides such as calcitonin gene-related peptide (CGRP). Local and systemic levels of CGRP increase rapidly during inflammatory responses. CGRP inhibits effector functions of various immune cells and dampens inflammation by distinct pathways involving the

Is mTOR involved in the mechanisms of the fast-acting antidepressant effects of AMPA receptor agonists?

LETTER TO THE EDITOR Yimin Hu • Xiangliu Liu • Jinchun Shen Received: 19 July 2012 / Accepted: 25 September 2012 / Published online: 11 October 2012 Springer-Verlag Wien 2012 We read with great interest the article ‘‘The involvement of AMPA-ERK1/2-BDNF pathway in the mechanism of new antidepressant action of prokinetic meranzin hydrate’’, in which the authors concluded that AMPA receptors were involved in the mechanism of the antidepressant effects of prokinetic meranzin hydrate (Xie et al. 2012). We appreciate the author’s excellent perspectives and want to

One-pot efficient synthesis of Na-urethane-protected b- and c-amino acids

SHORT COMMUNICATION Marta Cal • Mariusz Jaremko • Łukasz Jaremko • Piotr Stefanowicz Received: 14 June 2012 / Accepted: 1 December 2012 / Published online: 19 December 2012 The Author(s) 2012. This article is published with open access at Springerlink.com Abstract 1-[(4-Methylphenyl)oxy]pyrrolidine-2,5-dione and 1-[(4-methylphenyl)oxy]piperidine-2,6-dione react in a Lossen-type reaction with primary alcohols in the presence of triethylamine to furnish corresponding Na-urethaneprotected b-alanine and c-aminopropionic acid (GABA), respectively, with excellent yields and purities, in an

Catalytic mechanism of serine racemase from Dictyostelium discoideum

ORIGINAL ARTICLE Tomokazu Ito • Motoki Maekawa • Shuhei Hayashi • Masaru Goto • Hisashi Hemmi • Tohru Yoshimura Received: 1 September 2012 / Accepted: 30 November 2012 / Published online: 27 December 2012 Springer-Verlag Wien 2012 Abstract The eukaryotic serine racemase from Dictyostelium discoideum is a fold-type II pyridoxal 50-phosphate (PLP)-dependent enzyme that catalyzes racemization and dehydration of both isomers of serine. In the present study, the catalytic mechanism and role of the active site residues of the enzyme were examined by site-directed mutagenesis.

A cysteine in the repetitive domain of a high-molecular-weight glutenin subunit interferes with the mixing properties of wheat dough

ORIGINAL ARTICLE Xin Gao • Qisen Zhang • Marcus P. Newberry • Ken J. Chalmers • Diane E. Mather Received: 6 October 2012 / Accepted: 30 November 2012 / Published online: 12 December 2012 Springer-Verlag Wien 2012 Abstract The quality of wheat (Triticum aestivum L.) for making bread is largely due to the strength and extensibility of wheat dough, which in turn is due to the properties of polymeric glutenin. Polymeric glutenin consists of highand low-molecular-weight glutenin protein subunits linked by disulphide bonds between cysteine residues. Glutenin

Pressor response to L-cysteine injected into the cisterna magna of conscious rats involves recruitment of hypothalamic vasopressinergic neurons

ORIGINAL ARTICLE Yumi Takemoto Received: 5 July 2012 / Accepted: 28 November 2012 / Published online: 14 December 2012 Springer-Verlag Wien 2012 Abstract The sulfur-containing non-essential amino acid L-cysteine injected into the cisterna magna of adult conscious rats produces an increase in blood pressure. The present study examined if the pressor response to L-cysteine is stereospecific and involves recruitment of hypothalamic vasopressinergic neurons and medullary noradrenergic A1 neurons. Intracisternally injected D-cysteine produced no cardiovascular changes, while L-cysteine

Fast mass spectrometry-based enantiomeric excess determination of proteinogenic amino acids

ORIGINAL ARTICLE Heidi Fleischer • Kerstin Thurow Received: 30 September 2011 / Accepted: 28 November 2012 / Published online: 12 December 2012 Springer-Verlag Wien 2012 Abstract A rapid determination of the enantiomeric excess of proteinogenic amino acids is of great importance in various fields of chemical and biologic research and industries. Owing to their different biologic effects, enantiomers are interesting research subjects in drug development for the design of new and more efficient pharmaceuticals. Usually, the enantiomeric composition of

Biocatalysts for multicomponent Biginelli reaction: bovine serum albumin triggered waste-free synthesis of 3,4-dihydropyrimidin-2-(1H)-ones

ORIGINAL ARTICLE Upendra K. Sharma • Nandini Sharma • Rajesh Kumar • Arun K. Sinha Received: 25 September 2012 / Accepted: 27 November 2012 / Published online: 18 December 2012 Springer-Verlag Wien 2012 Abstract Bovine serum albumin (BSA) promoted simple and efficient one-pot procedure was developed for the direct synthesis of 3,4-dihydropyrimidin-2(1H)-ones including potent mitotic kinesin Eg5 inhibitor monastrol under mild reaction conditions. The catalyst recyclability and gram scale synthesis have also been demonstrated to enhance the practical utility of process.

Chiral bio-nanocomposites based on thermally stable poly (amide-imide) having phenylalanine linkages and reactive organoclay containing tyrosine amino acid

ORIGINAL ARTICLE Shadpour Mallakpour • Mohammad Dinari Received: 4 January 2012 / Accepted: 27 November 2012 / Published online: 11 December 2012 Springer-Verlag Wien 2012 Abstract Montmorillonite clay modified with the bioactive trifunctional L-tyrosine amino acid salt was used as a reactive organoclay (OC) for the preparation of poly(amide-imide) (PAI)/OC hybrid films. One of the functional groups of the L-tyrosine as the swelling agent formed an ionic bond with the negatively charged silicates, whereas the remaining functional groups were

Triple mutated antibody scFv2F3 with high GPx activity: insights from MD, docking, MDFE, and MM-PBSA simulation

ORIGINAL ARTICLE Quan Luo • Chunqiu Zhang • Lu Miao • Dongmei Zhang • Yushi Bai • Chunxi Hou • Junqiu Liu • Fei Yan • Ying Mu • Guimin Luo Received: 21 August 2012 / Accepted: 27 November 2012 / Published online: 6 December 2012 Springer-Verlag Wien 2012 Abstract By combining computational design and sitedirected mutagenesis, we have engineered a new catalytic ability into the antibody scFv2F3 by installing a catalytic triad (Trp29–Sec52–Gln72). The resulting abzyme, Se-scFv2F3, exhibits a high glutathione peroxidase (GPx) activity, approaching the native enzyme activity. Activity

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