Биохимия

Side reactions in the SPPS of Cys-containing peptides

ORIGINAL ARTICLE Panagiotis Stathopoulos • Serafim Papas • Charalambos Pappas • Vassilios Mousis • Nisar Sayyad • Vassiliki Theodorou • Andreas G. Tzakos • Vassilios Tsikaris Received: 19 September 2012 / Accepted: 12 February 2013 / Published online: 5 March 2013 Springer-Verlag Wien 2013 Abstract Alkylation of sensitive amino acids during synthesis of biologically important peptides is a common and well-documented problem in Fmoc-based strategy. Herein, we probed for the first time an unexpected S-alkylation of Cys-containing peptides that occur during

Senegalin: a novel antimicrobial/myotropic hexadecapeptide from the skin secretion of the African running frog, Kassina senegalensis

ORIGINAL ARTICLE Hui Wang • Renjie Li • Xinping Xi • Teng Meng • Mei Zhou • Lei Wang • Yingqi Zhang • Tianbao Chen • Chris Shaw Received: 24 November 2012 / Accepted: 11 February 2013 / Published online: 22 February 2013 Springer-Verlag Wien 2013 Abstract Amphibian skin is a rich and unique source of novel bioactive peptides most of which are endowed with either antimicrobial or pharmacological properties. Here, we report the identification and structural characterization of a novel peptide, named senegalin, which possesses both activities. Senegalin is a hexadecapeptide amide (FLPFLI

99mTc-labeled monomeric and dimeric NGR peptides for SPECT imaging of CD13 receptor in tumor-bearing mice

ORIGINAL ARTICLE Wenhui Ma • Fei Kang • Zhe Wang • Weidong Yang • Guiyu Li • Xiaowei Ma • Guoquan Li • Kai Chen • Yingqi Zhang • Jing Wang Received: 31 October 2012 / Accepted: 11 February 2013 / Published online: 1 March 2013 Springer-Verlag Wien 2013 Abstract CD13 receptor plays a critical role in tumor angiogenesis and metastasis. We therefore aimed to develop 99mTc-labeled monomeric and dimeric NGR-containing peptides, namely, NGR1 and NGR2, for SPECT imaging of CD13 expression in HepG2 hepatoma xenografts. Both NGR-containing monomer and dimer were

An expression system for the efficient incorporation of an expanded set of tryptophan analogues

ORIGINAL ARTICLE Dejan M. Petrovic ´ • Kees Leenhouts • Maarten L. van Roosmalen • Jaap Broos Received: 21 January 2013 / Accepted: 28 January 2013 / Published online: 13 February 2013 Springer-Verlag Wien 2013 Abstract Biosynthetic incorporation of tryptophan (Trp) analogues in recombinant proteins using an E. coli Trp auxotroph expression host is limited to analogues modified with a small substituent like a fluoro atom or a hydroxyl or amine group. We report here the efficient incorporation

WRF-TMH: predicting transmembrane helix by fusing composition index and physicochemical properties of amino acids

ORIGINAL ARTICLE Maqsood Hayat • Asifullah Khan Received: 10 October 2012 / Accepted: 23 January 2013 / Published online: 14 March 2013 Springer-Verlag Wien 2013 Abstract Membrane protein is the prime constituent of a cell, which performs a role of mediator between intra and extracellular processes. The prediction of transmembrane (TM) helix and its topology provides essential information regarding the function and structure of membrane proteins. However, prediction of TM helix and its topology is a challenging issue in bioinformatics and computational

S-linked protein homocysteinylation: identifying targets based on structural, physicochemical and protein–protein interactions of homocysteinylated proteins

ORIGINAL ARTICLE Yumnam Silla • Elayanambi Sundaramoorthy • Puneet Talwar • Shantanu Sengupta Received: 12 October 2012 / Accepted: 23 January 2013 / Published online: 12 February 2013 Springer-Verlag Wien 2013 Abstract An elevated level of homocysteine, a thiolcontaining amino acid is associated with a wide spectrum of disease conditions. A majority ([80 %) of the circulating homocysteine exist in protein-bound form. Homocysteine can bind to free cysteine residues in the protein or could cleave accessible cysteine disulfide bonds via thiol disulfide

Hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry for highly rapid and sensitive analysis of underivatized amino acids in functional foods

ORIGINAL ARTICLE Guisheng Zhou • Hanqing Pang • Yuping Tang • Xin Yao • Xuan Mo • Shaoqing Zhu • Sheng Guo • Dawei Qian • Yefei Qian • Shulan Su • Li Zhang • Chun Jin • Yong Qin • Jin-ao Duan Received: 14 August 2012 / Accepted: 15 January 2013 / Published online: 30 January 2013 Springer-Verlag Wien 2013 Abstract This work presented a new analytical methodology based on hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry in multiple-reaction monitoring mode for analysis of 24 underivatized free amino acids

Quinacrine reactivity with prion proteins and prion-derived peptides

ORIGINAL ARTICLE Zbigniew Zawada • Martin S ˇafar ˇ´ ık • Eva Dvor ˇa ´kova ´ • Olga Janous ˇkova ´ • Anna Br ˇezinova ´ • Ivan Stibor • Karel Holada • Petr Bour ˇ • Jan Hlava ´c ˇek • Jaroslav S ˇebestı ´k Received: 13 July 2012 / Accepted: 5 January 2013 / Published online: 23 January 2013 Springer-Verlag Wien 2013 Abstract Quinacrine is a drug that is known to heal neuronal cell culture infected with prions, which are the causative agents of neurodegenerative diseases called transmissible spongiform encephalopathies. However, the drug fails when it is applied in vivo. In this work, we

Fate of ferrisiderophores after import across bacterial outer membranes: different iron release strategies are observed in the cytoplasm or periplasm depending on the siderophore pathways

INVITED REVIEW Isabelle J. Schalk • Laurent Guillon Received: 28 December 2012 / Accepted: 9 February 2013 / Published online: 27 February 2013 Springer-Verlag Wien 2013 Abstract Siderophore production and utilization is one of the major strategies deployed by bacteria to get access to iron, a key nutrient for bacterial growth. The biological function of siderophores is to solubilize iron in the bacterial environment and to shuttle it back to the cytoplasm of the microorganisms. This uptake process for Gram-negative

Phylogenetic aspects of the sulfate assimilation genes from Thalassiosira pseudonana

INVITED REVIEW Mariusz A. Bromke • Rainer Hoefgen • Holger Hesse Received: 3 January 2013 / Accepted: 5 January 2013 / Published online: 26 January 2013 Springer-Verlag Wien 2013 Abstract Diatoms are unicellular algae responsible for approximately 20 % of global carbon fixation. Their evolution by secondary endocytobiosis resulted in a complex cellular structure and metabolism compared to algae with primary plastids. In the last years the interest on unicellular algae increased. On the one hand assessments suggest that diatom-mediated export production can influence climate

Страницы

Подписаться на Биохимия