Analysis of DNA interactions using single-molecule force spectroscopy

INVITED REVIEW Markus Ritzefeld • Volker Walhorn • Dario Anselmetti • Norbert Sewald Received: 30 January 2013 / Accepted: 13 February 2013 / Published online: 7 March 2013 Springer-Verlag Wien 2013 Abstract Protein–DNA interactions are involved in many biochemical pathways and determine the fate of the corresponding cell. Qualitative and quantitative investigations on these recognition and binding processes are of key importance for an improved understanding of biochemical processes and also for systems biology. This review article

The pentosidine concentration in human blood specimens is affected by heating

SHORT COMMUNICATION Masako Nakano • Midori Kubota • Shigeru Owada • Ryoji Nagai Received: 10 August 2011 / Accepted: 22 November 2011 / Published online: 4 December 2011 Springer-Verlag 2011 Abstract Pentosidine is an advanced glycation end product, formed by oxidation and glycation that accumulates markedly during end-stage renal failure. Measurement of the pentosidine level in physiological samples is applied as a sensitive marker for the early diagnosis of renal failure. In the quantitative measurements of pentosidine reported to date, a rapid enzyme-linked immunosorbent

Digestibility of extruded proteins and metabolic transit of Ne-carboxymethyllysine in rats

ORIGINAL ARTICLE Issam Alamir • Ce ´line Niquet-Leridon • Philippe Jacolot • Camille Rodriguez • Martine Orosco • Pauline M. Anton • Fre ´de ´ric J. Tessier Received: 28 July 2012 / Accepted: 2 November 2012 / Published online: 16 November 2012 Springer-Verlag Wien 2012 Abstract Milk proteins are frequently used as supplements in fortified foods. However, processing produces chemical changes which likely affect the nutritional advantage. This study was intended to explore the possible

Activation of the transcription factor Nrf2 in macrophages, Caco-2 cells and intact human gut tissue by Maillard reaction products and coffee

ORIGINAL ARTICLE Tanja Sauer • Martin Raithel • Ju ¨rgen Kressel • Gerald Mu ¨nch • Monika Pischetsrieder Received: 12 April 2011 / Accepted: 12 January 2012 / Published online: 25 January 2012 Springer-Verlag 2012 Abstract In addition to direct antioxidative effects, Maillard reaction products (MRPs) could increase the antioxidative capacity of cells through the induction of cytoprotective enzymes. Since many of those enzymes are regulated by the transcription factor Nrf2, the effect of MRPs on nuclear translocation of Nrf2 in macrophages and

Role of curcumin in the conversion of asparagine into acrylamide during heating

ORIGINAL ARTICLE Aytu ¨l Hamzalıog ˘lu • Burc ¸e A. Mogol • Roberta Barone Lumaga • Vincenzo Fogliano • Vural Go ¨kmen Received: 25 July 2011 / Accepted: 22 November 2011 / Published online: 6 December 2011 Springer-Verlag 2011 Abstract This study aimed to investigate the ability of curcumin to convert asparagine into acrylamide during heating at different temperatures. Binary and ternary model systems of asparagine–curcumin and asparagine–curcumin–fructose were used to determine the role of curcumin on acrylamide formation in competitive and uncompetitive

Effects of dietary bread crust Maillard reaction products on calcium and bone metabolism in rats

ORIGINAL ARTICLE Irene Roncero-Ramos • Cristina Delgado-Andrade • Ana Haro • Beatriz Ruiz-Roca • Francisco J. Morales • Marı ´a Pilar Navarro Received: 4 July 2011 / Accepted: 14 November 2011 / Published online: 23 November 2011 Springer-Verlag 2011 Abstract Maillard reaction products (MRP) consumption has been related with the development of bone degenerative disorders, probably linked to changes in calcium metabolism. We aimed to investigate the effects of MRP intake from bread crust on calcium balance and its distribution, and bone metabolism. During 88 days, rats were

Advanced glycation end products and diabetic retinopathy

REVIEW ARTICLE Ross Milne • Seymour Brownstein Received: 15 March 2011 / Accepted: 27 August 2011 / Published online: 11 September 2011 Springer-Verlag 2011 Abstract Retinopathy is a serious microvascular complication of diabetes and a major cause of blindness in young adults, worldwide. Early diabetic retinopathy is characterized by a loss of pericytes from retinal capillaries, the appearance of acellular capillaries and microaneurysms, and a breakdown of the blood–retinal barrier. In later stages, this can evolve into the proliferative phase in

Increasing effects of S-methyl-L-cysteine on the extracellular D-serine concentrations in the rat medial frontal cortex

SHORT COMMUNICATION Sayuri Ishiwata • Shigehiro Ogata • Asami Umino • Hirohisa Shiraku • Yoshitaka Ohashi • Yasushi Kajii • Toru Nishikawa Received: 6 September 2012 / Accepted: 23 January 2013 / Published online: 17 February 2013 Springer-Verlag Wien 2013 Abstract In an in vivo dialysis experiment, the intramedial frontal cortex infusion of a system A and Asc-1 transporter inhibitor, S-methyl-L-cysteine, caused a concentration-dependent increase in the dialysate contents of an endogenous coagonist for the N-methyl-D-aspartate (NMDA)

The use of native gels for the concomitant determination of protein sequences and modifications by mass spectrometry with subsequent conformational and functional analysis of native proteins following electro-elution

AMINO ACIDS PROTOCOLS Wei-Qiang Chen • Elena Karnaukhova • Gert Lubec Received: 15 February 2013 / Accepted: 18 February 2013 / Published online: 20 March 2013 Springer-Verlag Wien 2013 Abstract The protocol consists of running a native gel with in-gel digestion by proteases, subsequent mass spectrometrical determination of protein sequence and modifications, followed by electro-elution and conformational analysis using melting point and circular dichroism. Finally, the eluted protein is tested for preserved function.

Learning protein multi-view features in complex space

ORIGINAL ARTICLE Dong-Jun Yu • Jun Hu • Xiao-Wei Wu • Hong-Bin Shen • Jun Chen • Zhen-Min Tang • Jian Yang • Jing-Yu Yang Received: 13 June 2012 / Accepted: 13 February 2013 / Published online: 28 February 2013 Springer-Verlag Wien 2013 Abstract Protein attribute prediction from primary sequences is an important task and how to extract discriminative features is one of the most crucial aspects. Because single-view feature cannot reflect all the information of a protein, fusing multi-view features is considered as a promising route to improve prediction accuracy.


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